Progress in DNA sequencing

Recent progress in atomistic simulation of electrical current DNA sequencing.

We review recent advances in the DNA sequencing method based on measurements of transverse electrical currents. Device configurations proposed in the literature are classified according to whether the molecular fingerprints appear as the major (Mode I) or perturbing (Mode II) current signals. Scanning tunneling microscope and tunneling electrode gap configurations belong to the former category,...

Progress toward ultrafast DNA sequencing using solid-state nanopores.

BACKGROUND Measurements of the ionic current flowing through nanometer-scale pores (nanopores) have been used to analyze single DNA and RNA molecules, with the ultimate goal of achieving ultrafast DNA sequencing. However, attempts at purely electronic measurements have not achieved the signal contrast required for single nucleotide differentiation. In this report we propose a novel method of op...

Dna Sequencing In

A DNA sequencing method has been developed whereby DNA that has been cloned in a single-stranded bacteriophage vector can be sequenced from both ends. The method involves f i r s t making a minus-strand sense template from a single-stranded insert in the vector MJ3mp2 using a flanking primer~ and then sequencing the synthesized template using the d i d e o x y n u c l e o t i d e termination me...

DNA Sequencing

Double stranded DNA sequencing as a choice for DNA sequencing.

Double stranded DNA sequencing (1) is favored because of its simplicity, and convenice. However it is only recently that the quality of this method has become comparable with single stranded DNA sequencing. We believe that two factors have limited the popularity of double stranded DNA sequencing: 1, the quality of the template DNA; 2, the inherent propertv.,of the DNA polymerase. The modified T...